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The effluent was ionized via ion electrospray in negative mode on a TSQ Quantiva triple-quadrupole mass spectrometry with the following instrument settings: ion spray voltage 2500 V, sheath gas 28, ion transfer tube temperature 325, aux gas 8, and FWHM of 0.7 for both Q1/Q3 resolution

To isolate endothelial cells, pericytes, and tumor-infiltrating lymphocytes, single-cell suspensions in MACS buffer were mixed with CD31 beads, Thy-1 beads, or CD45 beads, respectively (all from Miltenyi Biotec) and incubated for 15 min on ice

CJC-1295 presses the GHRH receptor